Abstract
A fiber-optic biosensor based on the immobilization of chitinase enzyme on chitosan-solid waste tofu film has been proven to be effective for analyzing the presence of Cd (II) ions in the coastal area of Makassar. The working mechanism of the fiber optic biosensor is based on the inhibitory properties of the chitinase enzyme by the metal ion Cd (II). Research objectives determine the optimization of chitosan film by using solid waste tofu immobilized chitinase, characterization of chitosan film - solid waste tofu immobilized kiinase, the characterization of fiber optic biosensors includes inhibition percentage and measurement concentration range, detection limit value, selectivity and reproducibility. The results showed the optimum conditions for chitosan film-solid waste tofu at a concentration ratio of 55%: 45% with an intensity of 32.01, tensile strength of film (tensile strength) of 36.01. The characterization of the biosensor which includes the percentage of inhibition, and the measurement concentration range of Cd (II) ion was obtained at a percentage of 27.126% with a measurement concentration range of 1.0 x 10-5 – 1 x 10-8 M or equivalent to 0.0083 – 8.30 ppm (103 Cd (II) 83.0 ppm). The detection limit value is 6.1 or equivalent to 5.9 x 10-6 M (0.00076 ppm). The fiber optic biosensor is selective for Co (II), Cd (II) and Zn (II) ions with a biosensor reproducibility of 0.1592 + 0.0562. The measurement results for the concentration of metal ion Cd (II) at 4 (four) locations were in the range of 0.1446 – 0.270, the lowest was at the Losari Beach location at 0.1446 ppm while the highest was obtained at the Soekarno Hatta Port location at 0.2194 ppm. The fiber optic biosensor measurement did not differ significantly from the SSA
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