Abstract
In this paper we demonstrate for the first time the existence of an ether linkage between arabinogalactans type II (AGII) and a β(1,4)-xylan backbone. This polysaccharide fraction was isolated by ethanolic precipitation from a culture medium of Silene alba cells suspension. A combination of chromatographic methods, the use of a pure specific xylanase and the determination of the reducing end, has enabled us to establish the orientation of the linkage. The results were confirmed by methylation analysis assisted by mass spectrometry and by 13C-NMR spectroscopy. The proposed xylan structure is a β(1,4)-xylan backbone carrying AGII side chains of different sizes which are interspersed along the xylan chain. Such a result may be considered as an additional argument for supporting the importance of AGII in plant cell biology.
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