Abstract
<p>Endophytic <em>Streptomyces </em>sp. AEBg12 isolated from <em>Zingiber</em><em> </em><em>cassumunar</em><em> </em>(Bangle) is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by <em>Streptomyces </em>sp. AEBg12 and to assess the role of endophytic actinobacteria in producing pancreatic lipase inhibitor using endophytic-free bangle tissue culture, wild bangle and compared with the activity of <em>Streptomyces </em>sp. AEBg12 endophytes. Supernatant of <em>Streptomyces </em>sp. AEBg12 was extracted using ethanol, ethyl acetate, and n-hexane solvents. Toxicity test was performed using larvae of shrimp <em>Artemia</em><em> </em><em>salina</em>. The results showed that the best solvent to obtain pancreatic lipase inhibitor compounds was ethanol. Phytochemical analysis showed that ethanolic extract of endophytic <em>Streptomyces </em>sp. AEBg12 contained flavonoids. IC<sub>50</sub> value of ethanol extract was 180.83 µg/ml. The result of TLC showed that ethanolic extract of <em>Streptomyces </em>AEBg12 had a blue luminescence band indicated that there were either flavone, flavanones, flavonols or isoflavones. Inhibitory activity of <em>Streptomyces </em>sp. AEBg12 was higher than wild bangle and bangle tissue culture. The benefit of this study was to obtain new compound which might be used as an antiobesity drug through approach to the pancreatic lipase inhibitor.</p>
Highlights
Obesity is an abnormal excess of gained weight that occurs due to an excessive fat accumulation
This study aimed to discover the content of the compound, toxicity values, inhibition values, thin layer chromatography (TLC) of ethanolic extract of Streptomyces sp
The results showed that extraction with ethanol produced the highest yield (4.39 g) compared to other solvents, followed by ethyl acetate and n-hexane which were 0.48 and 0 g, respectively
Summary
Obesity is an abnormal excess of gained weight that occurs due to an excessive fat accumulation. It is the result of energy balance disorders where calories that enter the body are more than calories needed. Fat is not directly absorbed by the intestine unless it has been degraded by pancreatic lipase. Many study of this mechanism were performed to determine the effectiveness of natural products as antiobesity agents. Pancreatic lipase is an enzyme secreted by pancreas and contribute in fat digestion (Shin et al, 2003)
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