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Abstract
Rat hypophysial portal blood, collected from the pituitary stalk, was extracted and enkephalins were assayed by different RIA. Met-Enk-IR and Leu-Enk-IR levels were1635 ± 470pg/ml and125 ± 50pg/ml, respectively. Using HPLC characterization, the presence in portal blood of Met-Enk, Leu-Enk, proenkephalins fragments and dynorphin J-17 has been demonstrated. An unidentified Met-Enk-IR peptide has also been found.
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