Abstract
There aren’t a lot of studies about the bacterial communities associated with the Rosa canina and the aim of this study was to characterize endophytic bacteria from fruit of Rosa canina. The fruits of R. canina, which is growing wild in Slovakia, were collected in May 2013 from four locations: Nitra-Zobor, Vrbové-Baraní dvor, Rišňovce, Modra pažiť, Slovakia. Microbiological analyses were conducted by use of standard microbiological methods by spreading of fruits homogenates onto agar plate. Total viable count and mesophilic anaerobic sporulating bacteria were determined on Plate Count Agar after incubation for 2 days at 37 °C. Pseudomonas aeruginosa enumeration was carried out after incubation of Pseudomonas Isolation agar at 48 h at 35 °C. For members of the family Enterobacteriaceae (45 °C) Violet Red Bile Glucose agar were used and incubation was carried out for 24 h at 37 °C. For determinations of fungal colonies Malt agar and Czapek-Dox agar were inoculated using the spread-plate technique and incubated at 25 °C for 5 days. The yeasts were grown in Glucose Yeast Peptone agar (aerobiosis) at 25 °C during 72 hours. The total viable count of fruits ranged from 4.07 log cfu.g-1 in Rišňovce to 4.84 log cfu.g-1 in Vrbové Baraní dvor. Number of mesophilic anaerobic sporulating bacteria ranged from 4.09 in Vrbové Baraní dvor to 4.82 log cfu.g-1 in Modrá pažiť. Number of Pseudomonas aeruginosa count ranged from 2.00 in Nitra Zobor and Vrbové Baraní dvor to 3.94 log cfu.g-1 in Modrá pažiť. In our study the number of Enterobacteriaceae genera ranged from 3.38 in Nitra Zobor to 4.25 log cfu.g-1 in Vrbové Baraní dvor. Number of yeasts ranged from 3.36 in Vrbové Baraní dvor to 3.85 log cfu.g-1 in Modrá pažiť. Number of microscopic filamentous fungi ranged from 2.60 in Modrá Pažiť to 3.52 log cfu.g-1 in Nitra Zobor. Our findings indicate that Rose plant is naturally associated with a variety of endophytic microorganisms, which have different physiological and biochemical properties.
Highlights
The genus Rosa contains over 100 species that are widely distributed in Europe, Asia, the Middle East and North America
Despite the important role played by bacterial diversity in such plant communities, little is known on the distribution and abundance of endophytic bacteria in rose plant growing in such habitat
The outcome of this study will form the basis for the selection of endophytic bacteria that can be utilized for the facilitation of plant growth in such habitat
Summary
The genus Rosa contains over 100 species that are widely distributed in Europe, Asia, the Middle East and North America These deciduous shrubs are widely grown in gardens for their flowers and fruits. The endophytes often originate from the soil They initially infecting the host plant by colonizing, for instance, the cracks had been formed formed in lateral root junctions. It is generally assumed that many bacterial endophyte communities are the product of a colonizing process initiated in the root zone (Sturzet al., 2000), they may originate from other source than the rhizosphere, such as the phyllosphere, the anthosphere, or the spermosphere (Hallman, et al, 1997). The variations in the endophytic bacterial communities can be attributed to plant age, plant source, tissue type, time of sampling, and environment condition (Kobayashi and Palumbo, 2000). The aim of our study was to detect population of microorganisms on Rosa canina fruits from four different localities
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