Abstract
A custom-built ultrahigh-resolution optical coherence tomography (UHR-OCT) system and fluorescein staining were employed for investigation of a scopolamine induced dry eye mouse model. Acquired data was used to evaluate common and complementary findings of the two modalities. Central corneal thickness as measured by UHR-OCT increased significantly over the study period of 24 hours, from 89.0 ± 3.57 µm to 92.2 ± 4.07 µm. Both techniques were able to show corneal lesions with a large range of severity. Localized fluorescein staining was detected in 5% and diffuse staining in 45% of cases where no epithelial damage was visible with OCT. However, OCT revealed stromal defects in 6% and endothelial defects in 18% of the cases, which could not be visualized via fluorescein staining. Thus, while fluorescein staining widely detected defects of the corneal surface in a mouse model of dry eye disease, OCT non-invasively revealed additional information about defect depth and involvement of particular layers.
Highlights
Dry eye disease (DED) is a common disorder of the ocular surface that can impact negatively on patients’ quality of life [1] and cannot yet be treated at its underlying cause [2]
A custom-built ultrahigh-resolution optical coherence tomography (UHR-Optical coherence tomography (OCT)) system and fluorescein staining were employed for investigation of a scopolamine induced dry eye mouse model
Optical coherence tomography measurements were performed under isoflurane (2%) anesthesia applied via face mask
Summary
Dry eye disease (DED) is a common disorder of the ocular surface that can impact negatively on patients’ quality of life [1] and cannot yet be treated at its underlying cause [2]. The exact mechanisms of the disease are still unknown, but tear hyperosmolarity, tear film instability and a vicious cycle of ocular surface inflammations are accepted as defining causative factors [2,3]. Scopolamine is combined with a constant airflow chamber to induce desiccation. It has been classified as a combined tear film deficiency model, containing evaporation and aqueous deficiency as its causative factors [4]
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