Abstract
We sequenced and characterized the genes for homeodomain proteins (RrA1-hox1, RrA1-hox2) and the flanking mip gene in a bipolar ectomycorrhizal basidiomycete, Rhizopogon roseolus. The sequenced region mapped to the A mating type locus. By nucleotide alignment of partial nucleotide sequences of two Rrhox1 genes from two mating compatible monokaryotic strains, we deduced that the 3′-region of Rrhox1 gene sequences in R. roseolus has a divergence-homogenization duality, which makes restriction fragment length polymorphism (RFLP) analysis of this region possible. Using a primer pair designed according to the 3′-regions of the RrA1-hox1 and mip genes in the A1 mating type, target PCR products were amplified from ten dikaryotic strains of R. roseolus collected from nine prefectures in Japan. RFLP patterns obtained using AluI and RsaI allowed distinguishing of all ten R. roseolus strains, indicating that the 3′-regions of Rrhox1 and mip are suitable for strain identification.
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