Abstract
Tumor suppressor gene silencing by cytosine methylation contributes to cancer formation. Whether DNA demethylation enzymes counteract this oncogenic effect is just to be revealed. We have demonstrated that the dioxygenase TET1, a DNA demethylation enzyme which converts 5‐methylcytosine to 5‐hydroxymethylcytosine, 5‐formylcytosine and 5‐carboxylcytosine, is an important suppressor of tumor malignancy (Best 5 Article of Cell Reports, 2012). TET1 mRNA expression is significantly low in cancer tissues and its low abundance correlates with poor breast cancer patient survival. Understanding the upstream regulatory mechanism of TET1 expression might be important to control cancer progression. Here we constructed serial deletion mutants of TET1 promoter and identified a specific region to account for the high promoter activity in normal breast cells but low in malignant cancer cell lines.
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