Characterization of distal lymphoid nodules in the chicken caecum

Abstract

In this report, we describe lymphoid nodules consistently found in the distal region of each chicken caecum at approximately 3 cm from the ileo-cecal junction. These structures were studied by light microscopy both in normal and Eimeria tenella-infected chickens. They were observed with the naked eye in infected birds but not in normal chickens. In these latter the region of the caecal lamina propria corresponding to that in which the lymphoid aggregates were visible revealed a light infiltration by diffuse lymphoid tissue as well as a few germinal centers. The distal lymphoid nodules were studied using a panel of monoclonal antibodies which are specific for chicken Ig-containing cells, macrophages, Ia-like positive cells, and interdigitating cells, as well as a policlonal antiserum reactive with S-100 protein to stain both interdigitating cells and follicular dendritic cells. The immunohistochemical study demonstrated the resemblance of these aggregates to the caecal tonsils, suggesting that they represent specialized mucosa-associated lymphoid tissue that respond to antigens in the caecal lumen, their function being to enhance the mucosal defense provided by the caecal tonsils against antigens in the lumen of the caeca.