Characterization of Direct Readout Contacts of the Myb DNA-Binding Domain

Abstract

ABSTRACTThe Myb protein contacts its recognition sequence by means of direct protein–DNA interactions. We used site-directed mutagenesis in order to substitute amino acids crucial for these contacts and probed the mutant proteins for their DNA-binding and transactiving activities. We could show that amino acids involved in direct readout contacts do not contribute equivalently in the recognition process.