Characterization of cuticular proteins in CPR family in the wolf spider, Pardosa pseudoannulata, and the response of one subfamily genes to environmental stresses

Abstract

Cuticular protein (CP) plays an essential role in the construction and function of exoskeleton in arthropods. CPR family, CP with Rebers and Riddiford (R&R) Consensus, is the largest CP family in insects, but it lacks systematic research in non-insect arthropods. In this study, we explored CPRs in the wolf spider, Pardosa pseudoannulata, a predator to many insect pests. We totally identified 152 CPRs in P. pseudoannulata genome, which were divided into two subgroups based on R&R Consensus sequences, with 12 CPRs in RR-1 and 140 in RR-2. All RR-2 members presented a novel Consensus with 34 amino acids, G-x(8)-G-x(6)-Y-x-A-x(3)-G-x(7)-N-E-x-G, which was a common characteristic for RR-2 CPRs in chelicerates. Transcriptome data was used to document the expression patterns of CPR genes in different tissues and ecdysis processes. The specific expressions were found for part CPR genes, such as five RR-2 genes that were specifically expressed in male genital bulbs and eleven RR-1 genes that were highly expressed in the integument. Due to the limited number and integument-specific expression of RR-1 genes, we further analyzed their responses to different environmental stresses at the transcriptional level. Except for PapsCPR11, ten RR-1 genes responded to at least one environmental stress, among with the expression of PapsCPR12 was significantly changed by three stresses (dryness, low temperature and imidacloprid treatments). Silencing PapsCPR12 increased the tolerance of P. pseudoannulata to imidacloprid. Overall, the results presented novel Consensus characteristics of CPRs in P. pseudoannulata, which was helpful for the identification and evolution analysis of CPRs in non-insect arthropods.