Abstract
This study aimed to investigate collagen (Coll-I, III, IV) and elastin in canine normal prostate and prostate cancer (PC) using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle, and 5% surrounding blood vessels, in both normal prostate and PC. There was a higher median area of Coll-III in PC when compared to normal prostatic tissue (p = 0.001 for PSR and p = 0.05 for IHC). Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and nerve fibers of normal and PC samples. Although there was no difference in Coll-IV area between normal tissue and PC, tumors with Gleason score 10 showed absence of Coll-IV, when compared to scores 6 and 8 (p = 0.0095). Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples and were statistically higher in PC compared to normal tissue (p = 0.00229). Investigation of ECM components brings new information and should be correlated with prognosis in future studies.
Highlights
Cancer is the second leading cause of mortality worldwide
Formalin-fixed paraffin-embedded (FFPE) samples from canine prostatic tissue were sectioned for histological diagnosis, which was performed by three pathologists (LGRC, CEFA, PEK), at the same time, in a multi-head microscopy
There was no statistical difference of collagens fibers present red-orange birefringence (Coll-I) expression between normal and prostate cancer (PC) samples
Summary
Prostate cancer (PC) is the third most common malignant neoplasia (after non-melanoma skin cancer and lung cancer) [1]. Human PC often shows metastasis to bones and resistance to anti-androgen treatment [2]. Dogs spontaneously develop PC [3,4]. PC it is a very aggressive and highly metastatic disease [5]. Bone metastasis is diagnosed at a late stage of highly aggressive tumor subtypes [3]. Due to similarities in the clinical and pathologic aspects of PC in both species, some authors suggest that the dogs may be considered a good model for the study of human PC [4,6,7]
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