Abstract
A new variant of the blaOXA-546 gene, namely blaOXA-894, was identified on the chromosome of Shewanella xiamenensis isolated from pig wastewater in rural China. OXA-894 differs from OXA-546 (A46V, I219del) and OXA-48 (T167I, I219del) with two amino acid substitutions, respectively. The isolate was resistant to ampicillin, aztreonam, imipenem, meropenem and fosfomycin. Carba NP test confirmed S. xiamenensis strain sx20 as a carbapenemase-producer. The blaOXA-894 gene was located between the gene encoding a LysR family transcriptional regulator and the C15 gene. Its gene environment was similar to other S. xiamenensis with chromosome-located blaOXA-48-like genes. The T24H and T94V amino acid substitutions of LuxS protein were predicted to be deleterious, which may affect the virulence phenotype. The occurrence and potential health risk of carbapenem-resistant S. xiamenensis in a water environment is of concern.
Highlights
The blaOXA-48 gene, encoding class D beta-lactamases, was first reported in Klebsiella pneumoniae from a patient with urinary tract and skin burns in Turkey in 2001 [1]
Carbapenem minimum inhibitory concentrations (MICs) of S. xiamenensis strain sx20 were similar to S. xiamenensis IR34 harboring blaOXA-204 gene and S. xiamenensis DDP1 harboring blaOXA-416 (Table 1), but higher than strains of IR24 and IR33 harboring blaOXA-48 gene and S12-harboring blaOXA-181 gene [12,13,22], indicating there may be additional mechanisms for regulating carbapenem resistance in S. xiamenensis
That the mutants of luxS gene may affect the virulence phenotype of the S. xiamenensis strain sx20. This is the first report of chromosome-mediated blaOXA-894 gene in S. xiamenensis
Summary
The blaOXA-48 gene, encoding class D beta-lactamases, was first reported in Klebsiella pneumoniae from a patient with urinary tract and skin burns in Turkey in 2001 [1]. The enzyme usually hydrolyzed penicillins at high levels, but hydrolyzed carbapenems at a low level [2]. BlaOXA-48 -like genes were widely reported among K. pneumoniae and other Enterobacteriaceae [3]. 91 blaOXA-48 -like variants have been identified, with classical blaOXA-48 being the most widespread [4,5,6,7]. The number of reservoirs for these organisms was increasing among humans, animals and in the environment [3]. The rapid dissemination of carbapenem-resistant species harboring blaOXA-48 -like genes in different ecosystems has posed a severe threat to human health
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