Characterization of Chitin Synthase A cDNA from Diaphorina citri (Hemiptera: Liviidae) and Its Response to Diflubenzuron.

Abstract

Simple SummaryThe transcriptional level of chitin synthase A (DcCHSA) was investigated in Diaphorina citri Kuwayama at various developmental stages and tissues in this work. The survival and molting of D. citri nymphs were negatively impacted by high concentrations of DFB, which could also cause DcCHSA to express itself significantly. We also looked into how CHS knockout affected nymph development and eclosion. The findings revealed that DcCHSA was critical for the eclosion and molting of D. citri nymphs. This study provides a solid theoretical foundation for D. citri management in China.Diaphorina citri Kuwayama is the vector of HLB and one of the most common pests in citrus orchards in southern China. One of the most significant genes in D. citri’s growth and development is the chitin synthase gene. In this study, the CHS gene (DcCHSA) of D. citri was cloned and analyzed by bioinformatics. According to RT-qPCR findings, DcCHSA was expressed at many growth processes of D. citri, with the greatest influence in the fifth-instar nymph. The molting failure rate and mortality of D. citri rose as DFB concentration increased in this research, as did the expression level of DcCHSA. Feeding on DcCHSA caused a large drop in target gene expression, affected nymph molting, caused failure or even death in freshly eclosion adults, increased mortality, and reduced the molting success rate over time. These findings showed that DcCHSA was involved in nymph to adult development and may aid in the identification of molecular targets for D. citri regulation. It provided new ideas for further control of D. citri.