Characterization of Chicken Portomicron Remnant and Very Low Density Lipoprotein Remnant

Abstract

Portomicron remnant and very low density lipoprotein (VLDL) remnant in chickens were prepared by the method using ultracentrifugation (d<1.019) following an in vitro incubation of portomicron or VLDL prepared from chicken plasma with chicken lipoprotein lipase (LPL) for 2h at 37°C. The portomicron remnants and VLDL remnants yielded the almost single peak in the elution pattern on Bio-Gel A50m column (1.6×50cm) and the particle sizes were smaller than portomicron and VLDL since the elution of remnants by Bio-Gel A50m was similar to LDL rather than portomicron and VLDL. Chicken remnants had low triacylglycerol contents with high cholesterol and lower apolipoprotein C contents compared to portomicron or VLDL. These findings present the possible procedure to prepare in vitro chicken LPL-generated portomicron- and VLDL-remnants and suggested that the chicken remnants are characterized by a peculiar apolipoprotein composition while resemble to mammalian remnants in the lipid composition and particle size.