Abstract
A chemolithoautotrophic growth bacterium KP1-50T was isolated from 22-year-old volcanic deposits of Miyake-jima island (Japan) in this study. Its chemolithoautotrophic growth was tested using mineral salt medium (MS) medium under the atmosphere of H2, O2 and CO2. Phenotypic tests were mainly done with API 20NE kit system (bioMérieux). Phylogenetic position was determined by constructing the system tree. Chemolithoautotrophic growth of strain KP1-50T was proved by its growth curve with OD660 value of 0.5. The phylogenetic analysis based on 16S rDNA gene sequences indicated that sequence similarity between this strain and Herbaspirillum seropedicae LMG 6513T was 97%. The major cellular fatty acids are C16:0(31.3%), C16:1w7c/C15:0 2-OH iso (26.5%), C18:1 iso/trans (22.7%). The DNA G+C content of strain KP1-50T was 58mol%. DNA-DNA relatedness between strain KP1-50T and H.seropedicae LMG6513T, H.putei NBRC102406T and H.rubrisubalbicans NBRC102523T were 14.7%, 22.7% and 12.5% respectively. Strain KP1-50T seemed to be a novel species of the genus Herbaspicrillum, its accession number was AB366195 and the name of Herbaspirillum chemorialis sp. nov. was proposed.
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