Characterization of Cellulase Enzyme Produced by Two Selected Strains of Streptomyces Macrosporeus Isolated from Soil in Indonesia

Abstract

This study was conducted to characterize the cellulase enzymes produced by Streptomyces macrosporeus BB 32 and S. microspores KRC 21. D, which were isolated from Tanjung Pendam, Belitung Island, and from Cibodas Botanical Garden, Indonesia, respectively. The optimal activity of the enzymes was analyzed using parameters such as incubation time, pH, temperature, carboxymethylcellulose (CMC) concentration. The effect of the addition of some metal ions as activators or inhibitors was also analyzed spectrophotometrically at l 540 nm. Results demonstrated that the activity of the cellulase enzymes of S. macrosporeus BB 32 and S. macrosporeus KRC 21 D reached the optimum level after 2 and 5 days of incubation and at pH values of 8.0 and 6.0, temperatures of 35 °C and 50 °C, and CMC concentrations of 1.75% and 2%, respectively. S. macrosporeus BB 32 cellulase was activated by the cations CuCl 2 , MgCl 2 , and ZnCl 2 but inhibited by NaCl and CoCl 2 , reducing its activity. The cellulase of S. macrosporeus KRC 21.D was activated by the cation NaCl and by the divalent cations CoCl 2 , CuCl 2 , MgCl 2 , and ZnCl 2 . S. macrosporeus BB 32 was deposited at the Indonesian Culture Collection with the collection number InaCC A454.