Characterization of cDNAs encoding two glycine-rich proteins in chickpea ( Cicer arietinum L.): accumulation in response to fungal infection and other stress factors

Abstract

In chickpea plants infected with the pathogenic fungus Ascochyta rabiei [Pass.] Labr. several mRNAs for two glycine-rich proteins (GRPs) were identified by differential cDNA screening. The main part of the deduced amino acid sequences of the 14.6 kD GRP1 and the larger GRP2 consists of glycine-rich repetitive elements essentially as found for GRPs in other plants. Tyrosine residues in conserved positions inside these repetitive motifs suggest an involvement of the GRPs in a polymerization process by oxidative cross-linking, i.e. cell wall fortification. Both GRP transcripts are induced by infection with A. rabiei, showing a maximum of expression 5 days post infection. Wounding of leaves and the stress of water treatment (performed as a control) also seem to induce the accumulation of GRP transcripts.