Characterization of cDNAs encoding cytoplasmic ribosomal protein L15 and L27a in petunia (Petunia hybrida): primary structures and coordinate expression.

Abstract

We have isolated two cDNA clones, PhRL15 and PhRL27a, encoding cytoplasmic ribosomal proteins L15 and L27a, from a cDNA library prepared from petunia petal protoplast cultures. An expressed sequence tag (EST) strategy was employed. PhRL15 and PhRL27a contained open reading frames corresponding to proteins of 204 and 150 amino acids and molecular weights of 24,100 and 17,000Da, respectively. The deduced amino acid sequences of these clones are about 60% to 70%, identical with those of yeast and rat. Southern blot analysis indicates that each gene may be encoded by a small multigene. The transcription levels of both clones were high in young vegetative organs, at the early event of floral development, and in dividing petal protoplast, and relatively low in highly differentiated reproductive organs. Our results demonstrate that the expression levels of both clones are correlated with the rate of growth and coordinatively controlled in petunia plant.