Characterization of cadmium accumulation and phytoextraction in three species of the genus Atriplex (canescens, halimus and nummularia) in the presence or absence of salt

Abstract

This study aims to establish for the first time a comparison between the resistance to cadmium (Cd) stress of three halophyte species, Atriplex canescens, Atriplex halimus and Atriplex nummularia in addition to their already known tolerance for salt and drought. Plants were exposed to CdCl2 (20 and 50 μM) in the presence or in the absence of salt (50 mM NaCl) for one and two months. The amount of accumulated Cd was determined in the roots and leaves as well as the amount excreted on the surface of the leaves. Physiological parameters such as chlorophyll content and stress biomarkers, including malondialdehyde and enzymatic activities, were then analyzed. The results show that these plants are able to neutralize the excess of reactive oxygen species resulting from treatments by activating the antioxidant defense mechanisms in order to restore the homeostasis of cells.All three species are also able to accumulate high amounts of Cd in the leaves (several hundred mg of Cd/kg of dry leaves) and this phenomenon is amplified in the presence of salt. All together our results allow to consider the three Atriplex species as hyperaccumulators in the presence/absence of salt and as good candidates in a strategy of Cd phytoextraction in the presence of low concentrations of the pollutant. Nevertheless, both A. canescens and A. nummularia species seem to have a higher capacity to hyper-accumulate Cd when the concentration of Cd reaches higher level of contamination.