Abstract

: Twenty-nine strains from Fusarium section Liseola were screened for a zearalenone-like compound using analytical thin-layer chromatography (TLC). One metabolite had the same retention time on TLC and a similar multi-peak ultraviolet (UV) spectrum to zearalenone. Mass spectral and nuclear magnetic resonance (1H, 13C, 1H-13C heteronuclear multiple-quantum coherence) analysis gave information that helped identify the compound as the Fusarium pigment 8-O methyl bostrycoidin. The pigment was readily resolved from zearalenone with reversed-phase high-performance liquid chromatography analysis. Because this compound has a similar retention time, care should be exercised when using acid charring to visualize spots during zearalenone TLC analysis.

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