Abstract
AbstractSuitable and optimized chromatographic separation systems for HPLC analyses of mono‐, di‐ and oligomeric carbohydrates, organic acids (e.g. gluconic acid, α‐ketoglutaric acid), phospholipids (PE, LPE, LPC, PC, SPH) and neutral lipids (squalene, cholesterol) are demonstrated.Applications of HPLC technique are separation examples of sugars from hydrolyzed starches which were isolated from potatos, calculations of organic acids in fermentation mediums and determinations of neutral lipids and phospholipids which were isolated from microbial biomass.The liquid chromatographic separations are based on self‐packed highly efficient (approximately 80 000 theoretical plates per meter, N/m) glass columns.
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