Abstract
A significant need exists today to develop novel alternatives to traditional bone grafts. Here, we report the potential use of mechanochemically synthesized hydroxyapatite (HA), silicate-, or strontium-containing HA microparticles and microparticle aggregates in combination with polycaprolactone (PCL) as hybrid scaffolds for filling bone defects. The detailed characterization of scaffolds was performed with high-resolution synchrotron radiation–based microcomputed laminography, XRD, EDX, and FTIR. An in vitro cell-scaffold interaction analysis showed a significant improvement of cell spreading in the case of hybrid scaffolds with silicate- and Sr-containing HA. Scaffolds with Sr- and silicate-containing HA affected the expression of several genes involved in morphogenesis and transcription. Scaffolds with Sr-containing HA increased the expression of markers of the primary component of the extracellular matrix, and scaffolds with Sr-containing HA facilitated cell mineralization via an increase in osteocalcin production. The hybrid scaffolds with silicate- and Sr-containing HA microparticles exerted the highest antibacterial activity against gram-positive bacterium Staphylococcus aureus compared to the unmodified PCL scaffolds. Based on these findings, the obtained scaffolds with Sr- or silicate-containing HA are believed to hold promise for bone tissue regeneration as compared to scaffolds containing pure HA.
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