Characterization of Biofilms Formed by Foodborne Methicillin-Resistant Staphylococcus aureus.

Camino González-Machado,David Rodríguez-Lázaro,Martin Wagner,Vasilica Barbu,Marta Hernández,Elena Alexandra Oniciuc,Anca I. Nicolau,José María Eiros-Bouza,Carlos Alonso-Calleja,Rosa Capita,David Gallego

doi:10.3389/fmicb.2018.03004

Abstract

The objective of this study was to evaluate the capacity of 49 methicillin resistant Staphylococcus aureus (MRSA) from foods of animal origin (42 from dairy products and 7 from meat and meat products) to form biofilms. Overall, a higher biofilm biomass was observed for those MRSA strains harboring SCCmec type IV, while 8 MRSA strains (5 from dairy products and 3 from meat and meat products) were classified as strong biofilm formers in standard Tryptic Soy Broth medium. When a prolonged incubation period (48 h) was applied for those 8 MRSA strains, an increased biofilm biomass accumulation was observed during the time course, whereas the number of viable cells within the biofilms decreased as the biomass increased. The capacity of biofilm production correlated pretty well between the experiments using polystyrene microtiter plates and stainless steel micro-well plates, and significant higher values were observed in stainless steel when glucose was added to TSB during the enrichment. Biofilms were further characterized by confocal laser scanning microscope (CLSM), confirming that proteins and α-polysaccharides were the predominant components inside the extracellular polymeric matrix of biofilms formed by MRSA strains. In conclusion, our results confirm that MRSA isolates from foods of animal origin have significant capacity for forming biofilms with a high protein content, which can play a key role for the successful dissemination of MRSA lineages via food. Knowledge of the capacity of MRSA strains to produce biofilms, as well as characterization of the main MRSA biofilms matrix components, can help both to counteract the mechanisms involved in biofilm formation and resistance and to define more rational control strategies by using tailor-made cleaning agents.

Highlights

Methicillin-resistant Staphylococcus aureus (MRSA) has emerged since antimicrobial therapy was introduced in hospitals, having nowadays the ability to resist several classes of antibiotics (Lowy, 2003)
A higher biofilm biomass was observed for those methicillin resistant Staphylococcus aureus (MRSA) strains harboring SCCmec type IV (Table 1), being in accordance with previous studies that proved that such strains harboring SCCmec have greater probability to produce higher biofilm biomasses in comparison with those carrying SCCmec types I-III (Vanhommerig et al, 2014)
The number of viable cells within a MRSA-producing biofilm decreases as its biomass increases (Figure 3A), while the coefficient of variation calculated for the 48 h biofilms biomasses shows a low error between the different plotted biomasses (Figure 3B)

Summary

Introduction

Methicillin-resistant Staphylococcus aureus (MRSA) has emerged since antimicrobial therapy was introduced in hospitals, having nowadays the ability to resist several classes of antibiotics (Lowy, 2003). Singh et al (2010) showed diminished penetration of antibiotics such as oxacillin, cefotaxime or vancomycin into biofilms formed by S. aureus and Staphylococcus epidermidis. Those biofilms remained unaffected when amikacin and ciprofloxacin were introduced (Singh et al, 2010). Due to these reasons, the potential to cause infections in people with indwelling medical devices is highly increasing and as result, many studies have addressed this topic. A correlation between biofilm formation, their composition and molecular aspects of MRSA strains has been highlighted

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