Abstract
The importance of non-tuberculosis mycobacterial biofilm species in medicine, industry and the environment has recently gained attention. Our objectives were to characterize biofilm growth of Mycobacterium phlei M4, as a model of rapidly growing mycobacteria using the minimal biofilm eradication concentration (MBEC™) and to compare biocide susceptibility of planktonic and biofilm organisms. Scanning electron microscopy was also carried out to observe biofilm morphology. With the exception of Sporicidin® and Virkon® the minimum bactericidal concentration values for all biocides tested were lower than the MBEC values. The MBEC™ assay system was seen to produce multiple and reproducible biofilms of M. phlei and to be a useful tool for susceptibility studies.
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