Characterization of Bioactive Chemical Compounds from Staphylococcus aureus and Evaluation of Antibacterial Activity

Abstract

Aims and Objectives: This research aimed to analyze the bioactive chemical products of Staphylococcus aureus and evaluate the antibacterial and in vitro antimicrobial activities of plant extracts against Staphylococcus aureus. Method: The chemical components known as bioactives, which are sometimes referred to as secondary metabolites, were examined using gas chromatography-mass spectrometry (GC-MS) techniques. Subsequently, the antibacterial activity of the methanolic extract of Staphylococcus aureus was assessed in vitro. Results: The GC-MS analysis of Staphylococcus aureus detected the presence of the following: The compounds listed include ethyl 12-aminododecanoate, 1,11-Diaminoundecan-6-ol, N-propylidenehydroxylamine, 3,5-diamino-2,6-dicyanopyrazine, and methyl 1-methylpiperidine-3-carboxylate. The compound is called 5-azoniaspiro[4.5]decane. The compounds mentioned include 3,3-Dimethyl-2-acetyloxirane, 2-Hexadecanol, and 2-methyl-2-hexadecanol. The compounds mentioned are 12,15-octadecadiynoic acid and 9,11-octadecadiynoic acid. 8-oxo- 2-Ethyl-3,5-dimethylpyridine, 3,5-Dimethyl-2-ethylpyridine, ethyl 5,6-dimethylpyridine-3-carboxylate, 10-Oxododecanoic acid, and 12-Ethoxy-12-oxododecanoic acid. Glycyl-D-asparagine is a compound. 2,5-Piperazinedione, 3,6-bis(2-methylpropyl)-1H-pyrazin-2-one, and l-Leucyl-d-leucine. The evaluation of the antibacterial activity revealed that the metabolites of Staphylococcus aureus had a remarkably high level of activity against Escherichia coli (9.28±0.05). Equisetum arvense extract exhibited significant antimicrobial activity against Staphylococcus aureus.