Characterization of benzodiazepine receptors in primary cultures of fetal mouse brain and spinal cord neurons

Abstract

Primary cultures of fetal mouse brain and spinal cord were examined for the presence of binding sites for [ 3H]diazepam. Both brain and spinal cord cultures contain high affinity binding sites which resemble benzodiazepine receptors found in mammalian CNS with respect to both pharmacologic profile and response to exogenously applied GABA. These observations, coupled with the electrophysiologic properties of these cells suggest that primary cultures of fetal mouse brain and spinal cord may be valid models for studying the role and regulation of the benzodiazepine receptor.