Abstract
BackgroundATP-binding cassette (ABC) transporters constitute one of the largest transporter protein families and play a role in diverse biological processes.ResultsIn the present study, bacteriocin isolated from the Enterococcus casseliflavus MI001 strain was identified as an ABC transporter ATP-binding protein. The optimal conditions for the production of bacteriocin were found to be at 35 °C, a pH 5.5, and an incubation time of 24 h. Purification was performed using ammonium sulphate precipitation, gel filtration, and DEAE ion exchange chromatography. The bacteriocin was purified with an eightfold purification scheme resulting with a specific activity of 15,000 AU/mg. The NMR spectrum of purified bacteriocin revealed the presence of amino acids, namely lysine, methionine, cysteine, proline, threonine, tryptophan, and histidine. Further, the bacteriocin ABC transporter showed antimicrobial activity against food spoilage microorganisms.ConclusionsThe ABC transporter ATP-binding protein could be used as a potential alternative for food preservation, and it may be considered as a bio-preservative agent in food processing industries.
Highlights
Living organisms depend on various means of transport for the uptake of external nutrients and sequestration of waste products into the surrounding environment [3]
E. casseliflavus MI001 grown in MRS broth at 35 °C showed high antimicrobial activity against the indicator organisms Pseudomonas aeruginosa, S. aureus, and Escherichia coli after 24 h of incubation, which disappeared after 48 h of incubation
After an increase in temperature up to 40 °C for 24 h of incubation, bacteriocin production was at a minimum, as it showed a reduction in antimicrobial activity
Summary
Living organisms depend on various means of transport for the uptake of external nutrients and sequestration of waste products into the surrounding environment [3]. ATP-binding cassette (ABC) transporters are one of the largest super families of transport proteins present in all forms of life. They play diverse roles in both prokaryotes and eukaryotes. These transporters are the primary transporters functioning as both importers and exporters. Importers mediate the uptake of essential nutrients, vitamins, Mikkili et al Beni-Suef University Journal of Basic and Applied Sciences (2019) 8:5 A (a) (b) (c). ATP-binding cassette (ABC) transporters constitute one of the largest transporter protein families and play a role in diverse biological processes
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