Characterization of Bacterial Nanocellulose Produced by Tropical Fruit‐Derived Bacteria in Coconut Water Medium Supplemented with Pineapple Peel Extract

Abstract

AbstractAgro‐industrial residues, such as surplus fruits and vegetables are increasingly recognized as valuable sources for isolating bacterial strains capable of efficiently producing bacterial nanocellulose (BNC). This study presents a novel approach to BNC production using Komagataeibacter intermedius SB 110 isolated from rotten pineapple, cultivated in a sustainable medium of coconut water enriched with 5% sucrose and 0.5% pineapple peel extract. By leveraging agricultural waste, this method accelerates BNC biosynthesis to less than 7 days and achieves a yield of 5.563 g/L over 14 days, a 1.58‐fold increase compared with traditional media. The BNCs were primarily composed of cellulose type I, with minor cellulose type II, ultrafine fiber sizes (58.3 to 84.98 nm) and a high crystallinity index (60.81% to 78.34%), indicating superior structural quality. This innovative process not only demonstrates the potential of using low‐cost, sustainable substrates for high‐yield BNC production but also significantly enhances both the efficiency and scalability of the production process, setting a new standard over existing methods.