Abstract
Subclinical mastitis (SCM) is a major problem in the dairy industry creating a great loss both in quantity and quality of milk. The aim of this study to characterize bacteria, antibiogram profile, evaluation of immune cells and proinflammatory cytokines (IL-2, IL-6, TNF-α) associated with the inflammatory response in SCM. Out of total twenty six (26) milking cows, microbial and biochemical tests revealed that fifteen (15) cows (57.68%) having a total of 18 bacterial isolates of which the prevalence of coagulase negative Staphylococci spp., Staphylococcus aureus, Streptococci spp., E. coli, and Corynebacterium spp. were revealed as 38.89%, 16.67%, 22.22%, 16.67%, and 5.56%, respectively. On antibiogram profile, it was found effective against isolated organisms in ranking order are chlortetracycline, bacitracin, chloramphenicol, amoxicillin, nalidixic acid, penicillin, ampicillin, etc. although resistant isolates to penicillin, bacitracin, ampicillin, and chloramphenicol were detected. Blood and milk samples taken from those SCM+ cows further underwent for characterizing the pro-inflammatory cytokines IL-2, IL-6, and TNF-α, where both IL-2 and IL-6 revealed significant differences(P
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