Characterization of Bacterial Cellulose-Based Wound Dressing in Different Order Impregnation of Chitosan and Collagen.

Khatarina Meldawati Pasaribu,Syafruddin Ilyas,Tamrin Tamrin,Izabela Radecka,Saharman Gea

doi:10.3390/biom10111511

Khatarina Meldawati Pasaribu, Syafruddin Ilyas + Show 3 more

Open Access

https://doi.org/10.3390/biom10111511

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Abstract

Bacterial cellulose (BC), chitosan (Chi), and collagen (Col) are known as biopolymers which have met some properties that are required as wound dressing. This study focused on investigating the fabrication of BC-based wound dressing with chitosan and collagen, since chitosan has red blood cells binding and anti-bacterial properties, while collagen can support cell and tissue growth for skin wounds. The BC-based wound dressing was prepared by impregnating BC fibers in the chitosan and/or collagen solution for 24 h. FTIR was used to confirm the intermolecular interaction of amine and hydroxyl group of chitosan and/or collagen in BC-based wound dressing. Furthermore, the XRD diffractogram of the wound dressing show broader peaks at 14.2°, 16.6°, and 22.4° due to the presence of chitosan and collagen molecules in BC fibers. These results were then supported by SEM images which confirmed that chitosan and collagen were well penetrated into BC fibers. TGA curves revealed that BC/Chi/Col has better thermal properties based on the Tmax compare to BC/Col/Chi. Feasibility of the mats to be applied as wound dressing was also supported by other tests, i.e., water content, porosity, and hemocompatibility, which indicates that the wound dressing is classified as nonhemolytic materials. However, BC/Col/Chi was considered a more potential wound dressing to be applied compared to BC/Chi/Col since it has larger pores and showed better antibacterial properties (larger zones of inhibition) against S. aureus and E. coli via disk diffusion tests.

Highlights

Wounds, whether acute or chronic, can cause tissue damage and, require special treatment to stimulate the healing process
It can be observed that there was shifting and decreasing intensity of the peak in both the Bacterial cellulose (BC)/Chi/Col and BC/Col/Chi fabricated mats for wound dressing applications. This was consistent with previous research which demonstrated that the integration of collagen and chitosan may induce hydrogen bonds to form between the two molecules as shown in Figure 3, which shifted and reduced the intensity of amino and carbonyl peaks [38]
In BC/Chi/Col, it can be seen that the peak C-O-C intensity is higher than BC/Col/Chi, this indicates that the content of BC-based wound dressing is more influenced by the biopolymer, which was first impregnated into BC

Summary

Introduction

Whether acute or chronic, can cause tissue damage and, require special treatment to stimulate the healing process. Dressings should cover and protect the affected area, and create optimal environment at the wound site to help healing [4,5]. Conventional, barrier-type dressings such as gauze or cotton are designed to provide protection to the wounds from external trauma and contamination. These dressings do not actively enhance the healing process. Several studies carried out in the area of wound management, indicated that a good dressing must play an active role in the healing process [5,6,7,8]. The active wound dressing needs to effectively accelerate the healing time and should reduce pain [11,12]

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Conclusion