CHARACTERIZATION OF BACTERIA ASSOCIATED WITH PNEUMONIA IN BLACK BENGAL GOATS

Abstract

The present research work was undertaken for the characterization of the bacterial pathogens responsible for pneumonia in black Bengal goats. Nasal swab samples (n = 50) were collected from the pneumonic black Bengal goats in Mymensingh and Sirajgonj districts. Samples were inoculated onto nutrient agar, eosin methylene blue (EMB) agar, MacConkey agar, and blood agar media for isolation of bacteria. Identification of bacteria was performed by the Gram's staining method, cultural properties and biochemical tests. Antibiotic sensitivity of bacterial isolates was performed against 11 antimicrobial agents. Pasteurella spp were isolated from 25 cases, and Staphylococcus spp from 13 cases. Mixed infection caused by the Pasteurella spp and Staphylococcus spp. were recorded in 12 cases. Pasteurella spp produced whitish, opaque circular and translucent colonies on nutrient agar, smooth, convex, glistening colonies on EMB agar and no hemolysis on blood agar. Staphylococcus spp have shown gray white or golden yellowish colonies on nutrient agar. Golden yellow colonies without hemolysis or whitish colonies with hemolysis were also produced by Staphylococcus spp. on the blood agar media. Pasteurella spp were indole positive, MR-VP negative and ferment dextrose, sucrose and mannitol with the production of acid. The Staphylococcus spp were positive to MR-VP, coagulase and catalase reactions, negative to indole test and fermented five basic sugars with acid production. Results of cultural and biochemical tests supported that these two isolates belonged to P. multocida and S. aureus. P. multocida were highly sensitive to ciprofloxacin and resistant to penicillin. S. aureus found to be highly sensitive to erythromycin, tetracycline, enrofloxacin, and norfloxacin and less sensitive to amoxicillin. DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11215Bangl. J. Vet. Med. (2011). 9(1): 67-71