Abstract
In the present work, we studied the D-serine distribution and abundance in the microbial strains. The study was validated using chromatographic assays, biochemical and molecular techniques. The bacteria isolated from the pomace dumping soil site (bacteria id A1C1) showed maximum growth (O.D 600 of ~0.8) within 48h in the minimal salt media supplemented with L-serine. The isolated strain was identified as ‘Bacillus tequilensis’ through 16sRNA sequencing. The strain was quantified for D-serine content by using RP-HPLC. The D-serine concentration was calculated as 0.919±0.02 nM in the bacterial cellular fraction by using a standard curve plot and linear curve equation. Further, the recovery % was also calculated for the spiked samples which vary from 85 to 90%. These results demonstrate that Bacillus tequilensis A1C1 has D-serine content in the whole cell lysate of the cultivated cells showing industrial significance and implication of the isolated strain for the synthesis of D-serine-derived products.
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