Abstract
In this study apoptosis is characterized in a CHO cell line cultivated in both batch and continuous perfusion culture. Data is presented which shows that apoptosis can be induced in this cell line with camptothecin and detected using DNA gel electrophoresis and annexin-V & caspase-3 flow cytometric analysis. Time profiles of caspase-3 and DNA laddering gels indicate that apoptosis is the predominant form of cell death experienced by this cell line in batch culture. Several reported apoptosis inhibitors are evaluated for their ability to improve batch performance (NAC, suramin, cyclosporin A, Ac-DEVD-CHO, z-VAD-fmk), of which only one, z-VAD-fmk, is shown to significantly improve batch survivability. However z-VAD-fmk's effectiveness at improving continuous culture is less profound, possibly due to this cell lines ability to die by an alternative apoptotic pathway. Finally, reduced productivity at ultra-low feed rates n perfusion culture is shown to be correlated to the induction of apoptosis.
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