Abstract
The functional peptides from protein hydrolysates of various fishery sources have been identified such as antioxidant activity. The main intention of this study was purification and characterization of antioxidative peptide from black eelpout muscle. The antioxidative peptides were purified from black eelpout (Lycodes diapterus) muscle using different proteases. Antioxidant activity of black eelpout hydrolysates was evaluated using DPPH radical scavenging activity. Among six hydrolysates, the pepsin hydrolysate had the highest antioxidant activity compared to the other hydrolysates. Therefore, it was further purified and a peptide with seven amino acid residues of DLVKVEA (784 Da) was identified by amino acid sequence analysis. The EC50 value for scavenging DPPH radicals by purified peptide was 688.77 μM. Additionally, the purified peptide exhibited protective effect against DNA damage induces by oxidation in mouse macrophages (RAW 264.7 cells). The results of this study suggest that black eelpout muscle protein hydrolysate could potentially contribute to development of bioactive peptides in basic research.
Highlights
Free radicals are highly reactive species with their single and unbalanced electrons
Antioxidant activity of black eelpout muscle hydrolysates Black eelpout muscle protein hydrolysates were prepared by using commercial proteases including Alcalase, αchymotrypsin, Neutrase, papain, pepsin, and trypsin
The pepsin hydrolysate may contain bioactive compounds that could react with free radicals to transform them into more stable products and terminate the radical chain reaction
Summary
Free radicals are highly reactive species with their single and unbalanced electrons. The body is supported with several antioxidant defense systems where they can scavenge and transform ROS or free radicals into harmless species (Yeung et al 2002). Various antioxidant peptides have been isolated from marine organisms through enzymatic hydrolysis, including abalone muscle (Haliotis discus hannai Ino) and scallop (Patinopecten yessoensis) (Zhou et al 2012), threadfin bream surimi (Wiriyaphan et al 2012), croaker (Otolithes ruber) muscle (Nazeer et al 2012), sand eel (Lee et al 2011a, 2011b), sardinelle (Sardinella aurita) (Bougatef et al 2010), tuna liver (Je et al 2009), marine rotifer (Byun et al 2009), and algae protein waste (Sheih et al 2009)
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