Abstract
Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non-myeloid expression of antifungal CLRs is limited to MelLec on endothelial cells in murine mucosal tissues.
Highlights
The version presented here may differ from the published version
C-type lectin receptors (CLRs) constitute a family of pattern recognition receptors (PRRs), many of which are involved in immunity [1]
These receptors, Dectin-1, Dectin2, Mincle, MCL, and MelLec, recognize ligands in the fungal cell wall and are able to induce intracellular signaling directly through integral, or membrane associated, signaling domains or by association with a signaling partner [1]. Most of these “antifungal” CLRs are thought to be expressed by myeloid cells, but there is some evidence suggesting that these receptors are expressed on nonmyeloid cells including endothelial and epithelial cells [4–8]
Summary
Open Research Exeter makes this work available in accordance with publisher policies. The version presented here may differ from the published version. You are advised to consult the published version for pagination, volume/issue and date of publication
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