Abstract
We generated three monoclonal antibodies (mAbs) (2D7, 10C7 and 12A3) reactive to the α-chain of feline CD8 (fCD8) molecule. Further we showed that reference anti-fCD8 mAbs, FT2, 3.357 and vpg9 recognize the β-chain, α-chain and αβ-complex epitope, respectively. Flow cytometric analysis using these mAbs suggested that fCD8 α + β - cells were present in lymphocytes of spleen, but not significantly in those of thymus, lymph nodes and peripheral blood of normal kittens.
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