Abstract
L-Ribulose and L-ribose are two high-value unnatural sugars that can be biosynthesized by sugar isomerases. In this paper, an L-arabinose isomerase (BvAI) from Bacillus velezensis CICC 24777 was cloned and overexpressed in Escherichia coli BL21 (DE3) strain. The maximum activity of recombinant BvAI was observed at 45°C and pH8.0, in the presence of 1.0mM Mn2+. Approximately 207.2g/L L-ribulose was obtained from 300g/L L-arabinose in 1.5h by E. coli harboring BvAI. In addition, approximately 74.25g/L L-ribose was produced from 300g/L L-arabinose in 7h by E. coli co-expressing BvAI and L-RI from Actinotalea fermentans ATCC 43279 (AfRI). This study provides a feasible approach for producing L-ribose from L-arabinose using a co-expression system harboring L-Al and L-RI.
Published Version
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