Abstract

AbstractAn immobilized biocatalyst system for enzyme production using a thermophilic organism is described. Bacillus stearothermophilus (ATCC 29609) immobilized on Rohm and Haas IRA–938 ion exchange resin was utilized for the production of a highly thermostable α‐amylase in a bench scale airlift fermentor. The equilibrium between suspended bacterial cells and the resin fit a Langmuir‐type sorption isotherm. Cell and α‐amylase productivity data for free cell and immobilized cell continuous culture were compared as functions of the dilution rate. Cell growth rates for free and immobilized cells were consistent with the Monod kinetic model, α‐Amylase production for free cells was described by an unstructured model associated with cell growth. The immobilized cell systems gave higher α‐amylase productivity than the free cell system but did not achieve the productivities predicted by the unstructured kinetic model.

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