Abstract
A microorganism for biological control of sludge bulking was isolated from soil. The isolated microorganism was able to degrade the sheath of the Sphaerotilus natans causing a bulking of activated sludge. The isolated strain, FK3, was identified as a Terrabacter species by analysis of its 16S rRNA genetic sequence. Glucose (0.1%, w/v) and 0.1% yeast extract as carbon and nitrogen sources, respectively, were used in the medium for Terrabacter sp. FK3 growth and enzyme production. Sheath-degrading crude enzyme was purified from a Terrabacter sp. FK3 culture broth via ultrafiltration, followed by DEAE-cellulose chromatography. Although the enzyme activity was optimal at pH 8 and 30°C, the enzyme maintained stable activity below 50°C and in the pH range from 5–10. Sheath-degrading enzyme activity was accelerated by addition of Mg2+, Ca2+, and Fe2+, but inhibited by addition of Ag2+ or Hg2+. Catalysis was additionally promoted by addition of hydroxyurea, sodium azide, and iodoacetic acid, but inhibited by EDTA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of the Korean Society for Applied Biological Chemistry
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
