Abstract
Solution plasma process (SPP) was adopted to prepare alginate/silver nanoparticle (AL/AgNP) biocomposites. The biocomposites were synthesized in solutions of varying concentrations of AgNO3(1−5 mM) and alginate (0.1−0.3%, w/w) by discharging plasma for 7 min at 800 V with 30 kHz frequency using a pulsed unipolar power supply. The AL/AgNP emulsion was fabricated into 3D scaffolds by freeze drying and lyophilization and then stabilized by cross-linking via UV irradiation. UV-Vis spectroscopy of the biocomposites showed a characteristic absorbance at the maximum of 415–440 nm with increase in the intensity of the peaks as the concentration of AgNO3increased. FE-SEM analysis showed that the 3D scaffolds had microporous structures with fine and uniform pores of 3–9 ± 2.0 μm in diameter. TEM analysis revealed that AgNPs in the biocomposites were in spherical shape with size range of 5–40±2.0 nm (AL0.3/Ag5) and well distributed in the matrix. The AL/AgNP biocomposites showed microbicidal activity against 9 human pathogens with MIC of 9.6–21 μg/mL for bacteria and 85–425 μg/mL for fungi. Almost all of theE. colicells (99.8%) were killed by the treatment with 42.5 μg/mL of AgNPs at room temperature for 1 h.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
