Abstract
In the present study, Ag nanoparticles (NPs) were synthesized by eco-friendly method. Design and analysis of Taguchi experiments was done by the Qualitek-4 software. Effect of AgNO3 concentration (0.1, 0.01 and 0.001 M), incubation and culturing time (48, 72, 96 hours) as three different levels were measured as three major factors in NPs biosynthesis. We carried out biosynthesis of NPs through cell-free culture supernatant of Bacillus sp. HAI4. X-ray diffraction (XRD), Fourier Transform Infra-Red spectroscopy (FT-IR), Ultraviolet–Visible spectroscopy (UV) and Scanning Electron Microscopy (SEM) techniques are applied for NP characterization. It was demonstrated the average particles size was 33/264 nm with spherical shapes. Also, for evaluating of antibacterial effects, maximum zone of inhibition, two important multidrug resistant pathogenesis bacteria, Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 43300 were used. Three levels of Ag NPs had antibacterial effect on these bacteria (1, 1 and 0.5 cm for E.coli and 1.1, 1.1 and 1.1 for S.aureus) but there was no significant difference between three levels.
Highlights
Improving disinfection methods in order to fight against pathogenesis microorganisms multidrug resistant bacteria are important in hospital infections (Varshney et al, 2012)
Xray diffraction (XRD), Fourier Transform Infra-Red spectroscopy (FT-IR), Ultraviolet–Visible spectroscopy (UV) and Field Emission Scanning Electron Microscope (FESEM) techniques were applied for NP characterization
Three levels of Ag NPs concentrations had antibacterial effect on these bacteria (1, 1 and 0.5 cm for E.coli and 1, 1.1 and 1.1cm for S.aureus), but there was no significant difference between three levels
Summary
Improving disinfection methods in order to fight against pathogenesis microorganisms multidrug resistant bacteria are important in hospital infections (Varshney et al, 2012). Silver ions were common antibacterial that applied in catheters (Jain and Pradeep, 2005) In this case, with knowledge of bactericidal effect on E. coli and Bacillus subtilis, copper and silver ions have been used as antibacterial agents in wastewater of hospitals (Lin et al, 1996; Lin et al, 1998; Blanc et al, 2005; Yoon et al, 2007). With knowledge of bactericidal effect on E. coli and Bacillus subtilis, copper and silver ions have been used as antibacterial agents in wastewater of hospitals (Lin et al, 1996; Lin et al, 1998; Blanc et al, 2005; Yoon et al, 2007) These applications of ions have major disadvantages. These new functions of metal NPs are related to several properties such as surface area to volume ratio which are higher than conventional ion forms of metals (Vicky et al, 2010)
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