Abstract
BackgroundStem cells constitute a group of great capacity for self-renewal, long-term viability, and multi-lineage potential. Several studies have provided evidence that adipose tissue represents an alternative source of stem cells, with the main benefit of adipose-derived stem cells being that they can be easily harvested from patients by a simple minimally invasive method and can be easily cultured. The aim of this study was to establish a culture protocol for obtaining and characterizing adipose-derived stem cells (ADSCs) from C57BL/6 J mice.ResultsThe results showed that the yield, viability, and cell morphology obtained differ according to the age of isolated anatomic regions of the adipose tissue from ovarian and epididymis. The results of determination of cyclin D1 showed uniformity in the expression between different populations of ADSCs. A significant increase in the expression of caspase-3 active, was also observed in large cell populations from mice after 120 days. ADSCs were positive for mesenchymal markers CD90 and CD105, Nanog, SSEA-1, CD106, and VEGFR-1, and negative for hematopoietic markers CD34 and CD45. A large number of cells in S + G2/M phases was also observed for both sexes, demonstrating high proliferative capacity of ADSCs.ConclusionsWe observed that the adipose tissue of C57BL/6 J mice, isolated from the studied anatomic regions, is a promising source for obtaining pluripotent mesenchymal stem cells with high viability and proliferative response.
Highlights
Stem cells constitute a group of great capacity for self-renewal, long-term viability, and multi-lineage potential
During the initial days after plating, stem cells isolated from adipose tissue adhered to the surface of the culture plastic plates as a small cell population or polygonal spindle shaped forming a cells monolayer
The yield, morphology, and viability of cells obtained differed according to the age of tissue isolated from mice
Summary
Stem cells constitute a group of great capacity for self-renewal, long-term viability, and multi-lineage potential. Stem cells constitute a group of great capacity for selfrenewal, long-term viability, and multilineage potential [1]. The multilineage potential of embryonic and adult stem cells from bone marrow has been characterized extensively. In spite of the potential of embryonic stem cells for medical applications, many ethical and political issues accompany their use [2,3]. Stem cells therapy and ex vivo gene delivery have provided two promising strategies for treatment of a vast array of inherited and acquired disorders. For these types of therapy, a reliable source of stem cells appears necessary.
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