Abstract
Acid-soluble collagen was extracted from the skins of surf smelt and characterized. The yield of collagen was high about 24.0% on a dry weight basis. By SDS-polyacrylamide gel electrophoresis and CM-Toyopearl 650 M column chromatography, this collagen is a heterotrimer with a chain composition of α1α2α3. The denaturation temperature was 32.5℃, about 4.5℃ lower than that from porcine skin. Attenuated total reflectance-fourier transform infrared analysis showed that the percentage of secondary structural components in this collagen were 11% α-helix, 34% β-sheet, 19% β-turn, and 21% others. It suggests that the triple helical structure is present in the acid-soluble collagen from the skins of the surf smelt in comparison to that from the skin of porcine.
Highlights
Collagen has been extracted from the skins of vertebrate species such as pig and calf of bovine for industrial applications as functional foods, cosmetics, and biomedical materials
These materials cannot be used for the extraction of collagen, due to the outbreak of bovine spongiform encephalopathy (BSE), transmissible spongiform encephalophathy (TSE), foot-and-mouth disease (FMD) [1], and avian influenza which resulted in anxiety among the users of collagen and collagen-derived products from these land animals
We tried to extract and characterize the collagens from aquatic organisms because they have been reported not likely to be associated with infections such as BSE, TSE, and FMD, safe collagen samples can be obtained from aquatic organisms
Summary
Collagen has been extracted from the skins of vertebrate species such as pig and calf of bovine for industrial applications as functional foods, cosmetics, and biomedical materials. A high yield of collagen could be obtained from aquatic organisms, the physical and chemical properties of these collagens were different from those of land animals [2,3,4,5,6,7,8,9,10,11,12,13,14] These results indicated that collagens from aquatic organisms will have a potential to be important collagen sources for use in the food, cosmetic, and medical fields. We describe the isolation and physicochemical properties of acid-soluble collagen from the skins of surf smelt for use as an alternative to mammalian collagen in the food, cosmetic, and biomedical materials
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