Abstract
The isolation of acid-soluble collagen (ASC) from by-products of snakehead fish (Channa striata), including skin and the mixture of skin and scale, has been investigated. The recovery yield of fish skin ASC (13.6%) was higher than ASC from fish skin and scale (12.09%). Both ASCs were identified as type I collagen and showed maximal solubility at pH 2. Collagen samples from the mixture of skin and scale had higher imino acid content (226 residues/1000 residues) and lower wavenumber in the amide I and amide III region (1642 and 1203 cm−1, respectively) than the fish skin ASC (the imino acid content was 220 residues/1000 residues and the wavenumber in the amide I and amide III were 1663 and 1206 cm−1, respectively. The difference scanning calorimeter (DSC) showed higher thermal stability in ASC from the mixture of skin and scale (Td of 35.78 °C) than fish skin ASC (34.21 °C). From the result, the denaturation temperature of ASC had a close relationship with the content of imino acid as well as with the degradation of α-helix in amide I and III. These results suggest that collagen could be obtained effectively from snakehead fish by-products and has potential as a realistic alternative to mammalian collagens.
Highlights
The farming of snakehead fish, especially common snakeheads (Channa striata), a popular freshwater fish species, has rapidly spread in Vietnam [1]
Skin and the mixture of skin and scale of snakehead fish were collected from a dried snakehead fish and fish ball company located in An Giang province, Vietnam
The solution obtained after extraction was continuously treated with these main steps: (i) centrifuging at 9000× g for 20 min at 4 ◦ C to collect the supernatant; (ii) using NaCl for salt-out until obtaining a final concentration of 2.6 M in the presence of tris aminomethane 0.05 M at pH 7.0; (iii) centrifuging again at 9000× g for 20 min at 4 ◦ C to collect the precipitate; (iv) dissolving in 0.5 M acetic acid, dialyzing and lyophilizing at −20 ◦ C for 12 h; (v) collecting the acid-soluble collagen (ASC) by using a freeze-dryer
Summary
The farming of snakehead fish, especially common snakeheads (Channa striata), a popular freshwater fish species, has rapidly spread in Vietnam [1]. The main processed products from snakehead fish in Vietnam contained dried snakehead fish and fish balls. The main steps to produce dried snakehead fish were, removed scales, fillet, remove viscera, and skin. The main steps were to fillet and separate the fish meat and the mixture of skin and scale. The by-products discarded during processing mainly consisted of head, skin, bone, fins, scales, and viscera, and accounted for 54% of the snakehead fish weight [3]. The huge amount of these by-products caused serious ecological issues It was becoming a potential material for the production of value-added products, especially for the extraction of collagen and gelatin [4]
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