Abstract

The effects of hydrogen peroxide on normal and acatalasemic erythrocytes were examined. Severe hemolysis of acatalasemic erythrocytes and a small tyrosine radical signal (g = 2.005) associated with the formation of ferryl hemoglobin were observed upon the addition of less than 0.25 mM hydrogen peroxide. However, when the concentration of hydrogen peroxide was increased to 0.5 mM, acatalasemic erythrocytes became insoluble in water and increased the tyrosine radical signal. Polymerization of hemoglobin and aggregation of the erythrocytes were observed. On the other hand, normal erythrocytes exhibited only mild hemolysis by the addition of hydrogen peroxide under similar conditions. From these results, the scavenging of hydrogen peroxide by hemoglobin generates the ferryl hemoglobin species (H-Hb-Fe(IV)=O) plus protein-based radicals (*Hb-Fe(IV)=O). These species induce hemolysis of erythrocytes, polymerization of hemoglobin, and aggregation of the acatalasemic erythrocytes. A mechanism for the onset of Takarara disease is proposed.

Highlights

  • The effects of hydrogen peroxide on normal and acatalasemic erythrocytes were examined

  • We report that the oxidized hemoglobin species in the scavenging process were identified and that a novel property of acatalasemic erythrocytes was subjected to hydrogen peroxide treatment; erythrocytes aggregated to be insoluble in water at a high concentration (Ն0.5 mM) of hydrogen peroxide, whereas the severe hemolysis of acatalasemic erythrocytes resulted at low concentrations of hydrogen peroxide (Ͻ0.25 mM)

  • Asterisks indicate a significant difference from normal erythrocytes (*, p Ͻ 0.001)

Read more

Summary

EXPERIMENTAL PROCEDURES

Materials—Male mice (8 weeks old) of the C3H/AnLCsaCsa (normal) and C3H/AnLCsbCsb (acatalasemia) strains established by Feinstein et al [11] were maintained on a laboratory diet (MF diet, Oriental Yeast Co. Ltd., Tokyo, Japan) and water ad libitium until experiments. Chemicals of analytical grade were purchased from Sigma and Wako Pure Chemical Ind. General Procedures—Hemoglobin contents were determined by the method of Drabkin and Austin [12], and the concentrations of hemoglobin indicated were calculated as tet-

The abbreviations used are
RESULTS
DISCUSSION

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.