Characterization of a trinitrobenzene sulphonic acid (TNBS)‐induced mouse model of Periodontitis

Abstract

The 2,4,6‐trinitrobenzenesulfonic acid (TNBS)‐murine model has been used for immunopathogenesis of inflammatory bowel disease. The use of TNBS induces severe colonic inflammation when administered intrarectally in mice. The colitis which results from this procedure also presents clinical and histopathological findings common in periodontitis. This study will characterize parameters needed for successful induction of TNBS‐colitis as well as monitoring periodontal disease levels among 20 adult CD1 mice. The experimental groups were given 25 μL gingival injections of TNBS and the sham groups were given 25 μL saline solution (0.9% NaCl) in each quadrant of both maxillary and mandibular arches. Each group received 50 μL of TNBS or saline solution orally thrice weekly and was given both a gel diet in addition to solid mouse pellets. Oral and systemic tissue samples were collected at 2, 4, 6, 10 weeks. Radiographic examinations of both arches were taken to supplement histologic evaluations of changes in tooth supporting structures. Soft tissue samples were obtained from the large intestines and analyzed for the extent of systemic inflammation caused by TNBS. Colitis was present from second week on, with TNBS treatment. Radiographic and histochemical analyses revealed some changes in the alveolar bone pattern. Morphologic changes were noted in the colonic mucosa with extensive inflammatory infiltrates and some ulceration. This novel approach can help elucidate the relationship between alveolar bone loss and systemic manifestation common to gingival and periodontal disease for future therapeutic purposes.Grant Funding Source: PerioSciences, LLC