Abstract
Some properties and electron microscopy of an icosahedral RNA virus isolated from the soybean looper, Pseudoplusia includens, have been studied. The virus particles were 25 ± 1 nm in diameter, their sedimentation coefficient was 178 ± 4.2 S. and their buoyant density was 1.37 ± 0.01 g/cm 3. The RNA content was 37.9 ± 0.2% and the RNA was single stranded with a poly(A) track. The virus capsid contained three major proteins with molecular weights of 30.0 ± 0.8, 31.0 ± 0.9, and 34.0 ± 1.1 × 10 3, and two minor proteins with molecular weights of 33.0 ± 1.2 and 38.0 ± 1.1 × 10 3. One genome component was detected with molecular weight 3.3 ± 0.1 × 10 6. Agarose gel diffusion tests showed this virus has partial identity with cricket paralysis virus. Victoria strain. Electron microscopy revealed that high concentrations of virus particles were present in the midgut epithelial cells. Virus particles present in the lumen adjacent to these midgut epithelial cells appeared to have moved to the lumen from these cells. Virus particles could also be observed in the epidermal cells. Accumulations of microtubule and fibril containing vesicles in these cells appear to be due to the virus infection. It is proposed that this virus be included in the family Picornaviridae.
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