Abstract
A bacterial strain 2F5-2 capable of degrading phenanthrene was isolated from petroleum-contaminated soil.It was preliminarily identified as Sphingobium sp.according to its physiological biochemical characteristics and the analysis of its 16S rRNA gene sequence.Strain 2F5-2 could degrade 100% of 100 mg/L phenanthrene within 10 h.The optimal pH and temperature for the degradation were 7 and 30 ℃,respectively.The analysis of its phenanthrene-degrading pathway revealed that strain 2F5-2 degraded phenanthrene via salicylate pathway.The aromatic-ring-hydroxylating dioxygenases α-subunit encoding gene phdA was cloned and analyzed.It had 97.9%,98%,100% identity to the phdA gene from Sphingomonas sp.P2,Sphingobium yanoikuyae B1 and Sphingomonas sp.ZP1,respectively,indicating the conservation of this gene.Fig 6,Ref 16
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