Abstract
Oligosaccharides were prepared by alkaline cleavage and sodium borotritide reduction. Following gel filtration on Sephadex G-25, [ 3H]-oligosaccharides were further fractionated by anion-exchange and preparative paper chromatography. The principal neutral oligosaccharide contained GalNAcol:GlcNAc:Gal:Fuc (1:1:2:1). Using a combination of exoglycosidase digestion, periodate oxidation and methylation analysis by gas chromatography-mass spectrometry, it structure was determined to be: Gal β1→ 4 GlcNAc β1 → 3(Fuc α1 → 2 Gal 1 → 6)GalNAcol.
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